Two-Stage Pretreatment of Jerusalem Artichoke Stalks with Wastewater Recycling and Lignin Recovery for the Biorefinery of Lignocellulosic Biomass

Jerusalem artichoke (Helianthus tuberosus L.) is emerging as one of the energy plants considered for biofuel production. Alkali and alkali-involved pretreatment methods have been widely used bioconversion cellulosic materials due to their high sugar yield low inhibitor release. However, recovery treatment wastewater (black liquor) poorly studied. Here, we present a novel two-stage process design recycling black liquor. stalk (JAS) was first treated with 2% (w/v) NaOH, after which lignin recovered by H2SO4 at pH 2.0 from The recycled solutions were subsequently treat NaOH-pretreated JAS second time dissolve hemicellulose. CO-pretreated JAS, hydrolysates, acid-insoluble obtained above-mentioned pretreatment. A reducing 809.98 mg/g Co-pretreated achieved 48 h 5% substrate concentration using cellulase dosage 25 FPU/g substrate. In addition, hydrolysates containing xylose byproducts. strategy described here alkali acid combined provides an alternative approach biorefinery.